Affymetrix GeneChip Human Genome HG-U133A [HG-U133A]

Translational control plays a prominent role in the hepatocytic differentiation of HepaRG liver progenitor cells

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Comparison of transcriptional and translational regulation upon hepatocytic diffentiation by Total RNA and polysome bound RNA profiling.

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Transcription and translation profiling of human HepaRG cells upon hepatocytic differentiation

Comparison of transcriptional and translational regulation upon hepatocytic diffentiation by Total RNA and polysome bound RNA profiling.


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-Unknown

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-39434

The HepaRG cell line was cultured in William's E medium (Invitrogen) supplemented with 10% Fetal Calf Serum (Cellgro), 100 units/ml penicillin, 100 µg/ml streptomycin (Invitrogen), 5 µg/ml insulin (Sigma), and 5 x 10–5 M hydrocortisone hemisuccinate (Sigma). To induce differentiation, a two-step procedure was used [Parent R et al. Gastroenterology 2004; 126-1147-56]. Proliferative cells were harvested 2 days after seeding. Differentiated cells were harvested 28 days after seeding

P-MEXP-39438

P-MEXP-39439

P-MEXP-39442

GC-RMA (Robust Milti-chip Analysis) module from GeneTraffic 3.2.-11 (Iobion Stratagene Microarray Analysis Software) was used for normalization.

P-MEXP-51979

Proliferative cells have been harvested two days after seeding. Differentiated cells have been cultured for two weeks in standard Williams E medium and then for two additional weeks with standard Williams E medium supplemented with EGF and DMSO.

Transcription and translation profiling of human HepaRG cells upon hepatocytic differentiation

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