Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis.

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Arabidopsis plants were treated either with mock or MSB (0.2 mM of Menadione sodium bisulphite). Tissue was sampled after 3, 6 and 24 hours.

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Transcription profiling by array of Arabidopsis plants treated either with mock or menadione sodium bisulphite and sampled after 3, 6 and 24 hours

Arabidopsis plants were treated either with mock or MSB (0.2 mM of Menadione sodium bisulphite). Tissue was sampled after 3, 6 and 24 hours.

Arabidopsis plants were treated either with mock or MSB (0.2 mM of Menadione sodium bisulphite). Tissue was sampled after 3, 6 and 24 hours.

Affymetrix:Protocol:Hybridization-Unknown

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

Plants of Arabidopsis growing in short day were treated at day 17th of growth. The treatment was applied with a conventional sprayer. For mock treatments, only distilled water was used. For MSB, 0.2 mM of Menadione sodium bisulphite (SIGMA M5750) was used. The treatments were done approximately 30 minutes after the start of the artificial day.

The normalization was done with the package "affy" of "R". The function used was "rma(Data)".

Transcription profiling by array of Arabidopsis plants treated either with mock or menadione sodium bisulphite and sampled after 3, 6 and 24 hours

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Samples