Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis.

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Interaction of COP1 and UVR8, which regulate UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis thaliana

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Transcription profiling of Arabodpsis seedings exposed to UV-B irradiation

Interaction of COP1 and UVR8, which regulate UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis thaliana


Affymetrix:Protocol:Hybridization-Unknown

Affymetrix:Protocol:MAS4:ExpressionCall

Title: Affymetrix CHP Analysis (ExpressionCall). Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

Plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.

305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.

345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.

4 days under 345 nm cut-off +UV-B samples: 6h under 305 nm cut-off.

305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.

4 days under 345 nm cut-off +UV-B samples: 1h under 305 nm cut-off.

Transcription profiling of Arabodpsis seedings exposed to UV-B irradiation

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Samples