Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

Hormonal control of the shoot stem cell niche

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processedDataMatrix

Above ground tissue of 10 day old Arabidopsis seedlings of Col wild-type, 35S-ARR7, arr7, 35S-ARR15 was treated with Cytokinin (benzyladenine), Auxin (indole-3-acetic acid) or both.

GENOTYPE

TREATMENT

Transcription profiling by array of Arabidopsis mutant for arr7 and/or arr15 after treatment with cytokinin, auxin or both

Transcription profiling of Arabidopsis wild type, 35S-ARR7, arr7 and 35S-ARR15 treated with cytokinin, auxin or both

Above ground tissue of 10 day old Arabidopsis seedlings of Col wild-type, 35S-ARR7, arr7, 35S-ARR15 was treated with Cytokinin (benzyladenine), Auxin (indole-3-acetic acid) or both.


Affymetrix:Protocol:Hybridization-EukGE-WS2v4_450

Title: Affymetrix EukGE-WS2v4_450 Hybridization. Description:

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MTAB-4863

above ground tissue of 10 day old seedlings was harvested and incubated in 0.5 MS + 0.1% DMSO for 1 hour.

P-MTAB-4864

above ground tissue of 10 day old seedlings was harvested and incubated in 0.5 MS + 0.1% DMSO + 5µM BA for 1 hour.

P-MTAB-4865

P-MTAB-4866

above ground tissue of 10 day old seedlings was harvested and incubated in 0.5 MS + 0.1% DMSO + 5µM BA + 1µM IAA for 1 hour.

P-MTAB-4867

Raw data (Affy .CEL files) were quantile normalized, background corrected and transformed into expression estimates by gcRMA implemented in R. All settings were standard and all files were included in a single transformation. NOTE: expression estimates generated by gcRMA are log2 (log base 2) !

P-MTAB-4868

P-MTAB-4869

above ground tissue of 10 day old seedlings was harvested and incubated in 0.5 MS + 0.1% DMSO + 1µM IAA for 1 hour.

P-MTAB-4870

P-MTAB-4871

20 seedlings were co-treated and RNA was extracted from the pooled tissue.

Transcription profiling by array of Arabidopsis mutant for arr7 and/or arr15 after treatment with cytokinin, auxin or both

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