Comparison of R1 embryonic stem cells response to DMSO and retinoic acid and control

Transcription profiling of R1 embryonic stem cells treated with :to DMSO and retinoic acid vs control

Meta-analysis of Differentiating Mouse Embryonic Stem Cell Gene Expression Kinetics Reveals Early Change of a Small Gene Set

Affymetrix GeneChip Mouse Expression Array MOE430A [MOE430A]

Affymetrix GeneChip Mouse Expression Array MOE430B [MOE430B]

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processedDataMatrix

compound

Comparison of R1 embryonic stem cells response to DMSO and retinoic acid and control


Affymetrix:Protocol:Hybridization-EukGE-WS2v4

Title: Fluidics Station Protocol. Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-10095

Cytoplasmic RNA was extracted using the RNeasy mini kit (Qiagen)

P-MEXP-10096

Following 2 passages of growth on a layer of irradiated fibroblasts post thaw, R1 (p17) ESC lines were transferred to plastic tissue culture dishes coated with 0.1% porcine gelatin.  Cells were grown for 96h at 37C/5%CO2.

P-MEXP-10097

Cells were centrifuged and resuspended in differentiation or maintenance media before replating on tissue culture dishes coated with 0.1% porcine gelatin at a density of 80 - 1500 cells/cm2 depending on the day of harvest.  Differentiation media consisted of Dulbecco's Modified Eagles Media (DMEM) supplemented with 15% ESC maintenance screened fetal bovine serum (FBS), 0.1mM non-essential amino acids, 2mM glutamine, 100 U/mL penicillin, 100 ug/mL streptomycin and 100 uM monothioglycerol and 1% dimethylsulfoxide (DMSO)

P-MEXP-10267

Cells were centrifuged and resuspended in differentiation or maintenance media before replating on tissue culture dishes coated with 0.1% porcine gelatin at a density of 80 - 1500 cells/cm2 depending on the day of harvest. Differentiation media consisted of Dulbecco's Modified Eagles Media (DMEM) supplemented with 15% ESC maintenance screened fetal bovine serum (FBS), 0.1mM non-essential amino acids, 2mM glutamine, 100 U/mL penicillin, 100 ug/mL streptomycin and 100 uM monothioglycerol and 10 ng/mL leukemia inhibitory factor and 2 uM retinoic acid (RA)

P-MEXP-10268

Cells were centrifuged and resuspended in differentiation or maintenance media before replating on tissue culture dishes coated with 0.1% porcine gelatin at a density of 80 - 1500 cells/cm2 depending on the day of harvest. Differentiation media consisted of Dulbecco's Modified Eagles Media (DMEM) supplemented with 15% ESC maintenance screened fetal bovine serum (FBS), 0.1mM non-essential amino acids, 2mM glutamine, 100 U/mL penicillin, 100 ug/mL streptomycin and 100 uM monothioglycerol and 10 ng/mL leukemia inhibitory factor.

P-MEXP-10313

Standard RMA normalization as implemented in the Affy package version 1.5.8 from Bioconductor.

Transcription profiling of R1 embryonic stem cells treated with :to DMSO and retinoic acid vs control

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Samples