Affymetrix GeneChip Rat Expression Array RAE230A [RAE230A]

Affymetrix GeneChip Rat Genome 230 2.0 [Rat230_2]

Gadd45α: a novel diabetes-associated gene potentially linking diabetic cardiomyopathy and baroreflex dysfunction.

Identification of changes in gene expression in dorsal root Ganglia in diabetic neuropathy: correlation with functional deficits

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A study of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes

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Transcription profiling of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes

A study of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v4

Title: Fluidics Station Protocol. Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v5_450

(Parameters: Extracted product = total_RNA, Amplification = none)

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-15984

Temperature 20-22  C, Humidity 40-60%, Specified rat pathogen free conditions, 12 hours light/dark cycle, Water ad libitum, Nutrients Standard Rat Diet no.1 expanded (Ban Kingman), Housed in cages according to United Kingdom Home Office Regulations.

P-MEXP-15985

Male Wistar rats with starting weights of 280 - 320 g were maintained without any specific treatment for 4 weeks.  After 4 weeks, animals were anaesthetised with inhaled isoflurane, percutaneous electrodes were inserted near the sciatic nerve at the sciatic notch and achilles heel and sensory and motor nerve conduction velocities measured.  Animals were then exanguinated and the L4 and L5 dorsal root ganglia dissected and immediately frozen at -80C prior to RNA extraction.  These procedures were carried out in accordance with UK Home Office regulations, under project licence 40/2459.

P-MEXP-15986

Male Wistar rats with starting weights of 280 - 320 g were maintained without any specific treatment for 1 week.  After 1 week, animals were anaesthetised with inhaled isoflurane, percutaneous electrodes were inserted near the sciatic nerve at the sciatic notch and achilles heel and sensory and motor nerve conduction velocities measured.  Animals were then exanguinated and the L4 and L5 dorsal root ganglia dissected and immediately frozen at -80C prior to RNA extraction.  These procedures were carried out in accordance with UK Home Office regulations, under project licence 40/2459.

P-MEXP-15987

Male Wistar rats with starting weights of 280 - 320 g were maintained without any specific treatment for 8 weeks. After 8 weeks, animals were anaesthetised with inhaled isoflurane, percutaneous electrodes were inserted near the sciatic nerve at the sciatic notch and achilles heel and sensory and motor nerve conduction velocities measured. Animals were then exanguinated and the L4 and L5 dorsal root ganglia dissected and immediately frozen at -80C prior to RNA extraction. These procedures were carried out in accordance with UK Home Office regulations, under project licence 40/2459.

P-MEXP-15988

Male Wistar rats with starting weights of 280 - 320 g were made diabetic by a single intraperitoneal injection of 55 mg/kg streptozotocin dissolved in fresh saline after an overnight fast.  Diabetes was confirmed 3 days later by measurement of blood glucose taken from the tail vein.  4 weeks after streptozotocin injection animals were anaesthetised with inhaled isoflurane, percutaneous electrodes were inserted near the sciatic nerve at the sciatic notch and achilles heel and sensory and motor nerve conduction velocities measured. Animals were then exanguinated and the L4 and L5 dorsal root ganglia dissected and immediately frozen at -80C prior to RNA extraction. These procedures were carried out in accordance with UK Home Office regulations, under project licence 40/2459.

P-MEXP-15989

Male Wistar rats with starting weights of 280 - 320 g were made diabetic by a single intraperitoneal injection of 55 mg/kg streptozotocin dissolved in fresh saline after an overnight fast. Diabetes was confirmed 3 days later by measurement of blood glucose taken from the tail vein.  8 weeks after streptozotocin injection animals were anaesthetised with inhaled isoflurane, percutaneous electrodes were inserted near the sciatic nerve at the sciatic notch and achilles heel and sensory and motor nerve conduction velocities measured. Animals were then exanguinated and the L4 and L5 dorsal root ganglia dissected and immediately frozen at -80C prior to RNA extraction. These procedures were carried out in accordance with UK Home Office regulations, under project licence 40/2459.

P-MEXP-15990

Male Wistar rats with starting weights of 280 - 320 g were made diabetic by a single intraperitoneal injection of 55 mg/kg streptozotocin dissolved in fresh saline after an overnight fast. Diabetes was confirmed 3 days later by measurement of blood glucose taken from the tail vein. 1 week after streptozotocin injection animals were anaesthetised with inhaled isoflurane, percutaneous electrodes were inserted near the sciatic nerve at the sciatic notch and achilles heel and sensory and motor nerve conduction velocities measured. Animals were then exanguinated and the L4 and L5 dorsal root ganglia dissected and immediately frozen at -80C prior to RNA extraction. These procedures were carried out in accordance with UK Home Office regulations, under project licence 40/2459.

P-MEXP-15991

P-MEXP-15992

P-MEXP-15993

TRANPRTCL2797

Expression analysis was performed using RMAExpress (02 release) using the background correction and quantile normalisation options. In addition, scaling normalisation (target signal 100) using Affymetrix MAS 5.1 was done for the .CHP files submitted to this database.

Transcription profiling of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes

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