Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

Salicylic Acid-Independent ENHANCED DISEASE SUSCEPTIBILITY1 Signaling in Arabidopsis Immunity and Cell Death Is Regulated by the Monooxygenase FMO1 and the Nudix Hydrolase NUDT7

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Gene expression profiling leading to the identification of novel components in the EDS1/PAD4-regulated defence pathway

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Transcription profiling of Arabidopsis leading to the identification of novel components in the EDS1/PAD4-regulated defence pathwayabidopsis-Pst-eds1-pad4

Gene expression profiling leading to the identification of novel components in the EDS1/PAD4-regulated defence pathway


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v4

Title: Fluidics Station Protocol. Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-16944

Plants were soil grown in controlled environment chambers under a regime of 10 h light period at 150-200 uEinsteins m-2 sec-1, 23  C and 65 % relative humidity. Four-week-old plants were used for microarray experiments.

P-MEXP-16945

Pseudomonas syringae pv. tomato strain DC3000 expressing avrRpm1 or avrRps4 was infiltrated at 10 E7 cfu/ml into leaves. Mock infiltration experiments were performed using 10 mM MgCl2 solution.

P-MEXP-16954

Total RNA extracted using RNAwiz reagent (Ambion). cRNA was prepared following the manufacturer's instructions (www.affymetrix.com/support/technical/manual/expression_manual.affx).Labelled cRNA transcripts were purified using the Sample Cleanup Module (Affymetrix). Fragmentation of cRNA transcripts, hybridization and scanning of the high-density oligonucleotide microarrays (Arabidopsis ATH1 Genome Array, Affymetrix) were performed according to the manufacturer's GeneChip Expression Analysis Technical Manual.

Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7.

Transcription profiling of Arabidopsis leading to the identification of novel components in the EDS1/PAD4-regulated defence pathwayabidopsis-Pst-eds1-pad4

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