Affymetrix GeneChip Yeast Genome 2.0 Array [Yeast_2]

Functional Interactions between Sphingolipids and Sterols in Biological Membranes Regulating Cell Physiology

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determination of gene regulation by sterol and sphingolipid composition

Genotype

Transcription profiling of yeast mutants to determine gene regulation by sterol and sphingolipid composition

determination of gene regulation by sterol and sphingolipid composition


P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-TABM-2950

Nucleic Acid Extraction Method: Qiagen RNeasy Plus Mini Kit ; Nucleic Acid Type: total_RNA ; Nucleic Acid Cleanup: Qiagen RNeasy Mini Columns ; Amplification Method: Ambion MessageAmp II-Biotin Enhanced

P-TABM-2951

cDNA Synthesis: Ambion MessageAmp II-Biotin Enhanced ; cDNA Cleanup: Ambion MessageAmp II-Biotin Enhanced ; cRNA Synthesis: Ambion MessageAmp II-Biotin Enhanced ; cRNA Cleanup: Ambion MessageAmp II-Biotin Enhanced ; Spike Target Element: mRNA+Target ; Spiking Control: Affymetrix standard ; Dye: biotin

P-TABM-2952

Washing/Staining Procedure: Midi_Euk2v3 ; Washing/Staining Instrumentation: Fluidics Station 450

Transcription profiling of yeast mutants to determine gene regulation by sterol and sphingolipid composition

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