Role of beta-arrestin2 in response to intermittent or continuous parathyroid hormone (PTH) treatment.

Transcription profiling of mouse primary osteoblastic cells after intermittent of continuous treatment with parathyroid hormone to investigate the role of beta-arrestin2

Beta-arrestin2 regulates parathyroid hormone effects on a p38 MAPK and NFkappaB gene expression network in osteoblasts

Affymetrix GeneChip Mouse Genome 430A 2.0 [Mouse430A_2]

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Transcription profiling by array of mouse primary osteoblastic cells from arrb2 knockout treated with parathyroid hormone

Role of beta-arrestin2 in response to intermittent or continuous parathyroid hormone (PTH) treatment.


Cell/Tissue Separation Technique: microdissection ; Treatment Type: drug compound/small molecule ; Drug Compound/Small Molecule: water (vehicle) ; Treatment Delivery Method: in medium ; In vivo/vitro Treatment Details: 6h treatment every 48h during 15 days

Cell/Tissue Separation Technique: microdissection ; Treatment Type: drug compound/small molecule ; Drug Compound/Small Molecule: parathyroid hormone (PTH) ; Treatment Delivery Method: in medium ; In vivo/vitro Treatment Details: 6 hours treatment every 48 hours, during 15 days

Cell/Tissue Separation Technique: microdissection ; Treatment Type: drug compound/small molecule ; Drug Compound/Small Molecule: parathyroid hormone (PTH) ; Treatment Delivery Method: in medium ; In vivo/vitro Treatment Details: 48 hours treatment every 48 hours, during 15 days

Cell/Tissue Separation Technique: microdissection ; Treatment Type: drug compound/small molecule ; Drug Compound/Small Molecule: parathyroid hormone (PTH) ; Treatment Delivery Method: in medium ; In vivo/vitro Treatment Details: 6 hours every 48 hours, during 15 days

Nucleic Acid Extraction Method: phenol-based method ; Nucleic Acid Type: total_RNA ; Nucleic Acid Cleanup: Qiagen RNeasy Mini Columns ; Amplification Method: RNA polymerase - single round

cDNA Synthesis: Affymetrix GeneChip One-Cycle cDNA Synthesis Kit ; cDNA Cleanup: phenol extraction and precipitation ; cRNA Synthesis: Affymetrix GeneChip Expression 3'-Amplification Kit for IVT Labeling ; cRNA Cleanup: Qiagen RNeasy Mini Columns ; Spike Target Element: mRNA+Target ; Spiking Control: Affymetrix standard ; Dye: biotin

Washing/Staining Procedure: Mini_Euk2v3 ; Washing/Staining Instrumentation: Fluidics Station 400

Image Analysis Software: GCOS ; Image Analysis Algorithm: GCOS

Cell/Tissue Separation Technique: microdissection ; Treatment Type: drug compound/small molecule ; Drug Compound/Small Molecule: water (vehicle) ; In vivo/vitro Treatment Details: 6hours treatment every 48 hours, during 15 days

Cell/Tissue Separation Technique: microdissection ; Treatment Type: drug compound/small molecule ; Drug Compound/Small Molecule: water (vehicle) ; Treatment Delivery Method: in medium ; In vivo/vitro Treatment Details: 6 hours treatment every 48 hours, during 15 days

Transcription profiling by array of mouse primary osteoblastic cells from arrb2 knockout treated with parathyroid hormone

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