Affymetrix GeneChip Murine Genome U74A [MG_U74A]

Affymetrix GeneChip Murine 11K Genome [Mu11KsubA]

Affymetrix GeneChip Murine 11K Genome [Mu11KsubB]

The Utility of DNA Microarrays for Characterizing Genotoxicity

rawData

scan

Mouse lymphoma L5178Y cells are treated with Mitomycin C [CAS:50-07-7;CHEBI:27504] and harvested at 4 and 24 hours for analysis.

compound

dose

performer

time

Transcription profiling of mouse lymphoma L5178Y cells treated with Mitomycin C - ILSI-HESI Genotoxicity Study - Mitomycin

Mouse lymphoma L5178Y cells are treated with Mitomycin C [CAS:50-07-7;CHEBI:27504] and harvested at 4 and 24 hours for analysis.


P-TOXM-190

Mouse lymphoma L5178Y TK+/- cells were maintained in RPMI 1640 media supplemented with 2 mg/mL sodium bicarbonate, 10ug/mL folic acid, 1.5 mg/mL L-glutamine, 0.11 mg/mL sodium pyruvate, 0.5 mg/mL Pluronit F68 and 100 U/mL final concentration each of a pen/strep mixture all adjusted to a pH of 7.2.  Media was supplemented with 10% heat-inactivated horse serum for cell growth.

P-TOXM-193

100mL of a 5 x 10^5 cells/mL culture is exposed to a defined concentration of each test chemical (dissolved in DMSO for final concentration of <= 1%) and incubated for 4 hours at 37 degrees C.

P-TOXM-194

Cells are washed twice with growth media through centrifugation for 5 min at 1200xg and resuspension.  Pellets are rinsed twice in ice-cold Dulbecco's PBS and finally subjected to TRIzol extraction protocol.  Samples are stored in DEPC treated water.

Transcription profiling of mouse lymphoma L5178Y cells treated with Mitomycin C - ILSI-HESI Genotoxicity Study - Mitomycin

Submitter Supplied Information

Samples