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Accession IconGSE53514

Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells

Organism Icon Homo sapiens
Sample Icon 34 Downloadable Samples
Technology Badge Icon Affymetrix Human Gene 1.0 ST Array (hugene10st)

Submitter Supplied Information

Description
CD4(+) type 1 T regulatory (Tr1) cells are induced in the periphery and have a pivotal role in promoting and maintaining tolerance. The absence of surface markers that uniquely identify Tr1 cells has limited their study and clinical applications. By gene expression profiling of human Tr1 cell clones, we identified the surface markers CD49b and lymphocyte activation gene 3 (LAG-3) as being stably and selectively coexpressed on mouse and human Tr1 cells. We showed the specificity of these markers in mouse models of intestinal inflammation and helminth infection and in the peripheral blood of healthy volunteers. The coexpression of CD49b and LAG-3 enables the isolation of highly suppressive human Tr1 cells from in vitro anergized cultures and allows the tracking of Tr1 cells in the peripheral blood of subjects who developed tolerance after allogeneic hematopoietic stem cell transplantation. The use of these markers makes it feasible to track Tr1 cells in vivo and purify Tr1 cells for cell therapy to induce or restore tolerance in subjects with immune-mediated diseases.
PubMed ID
Total Samples
36
Submitter’s Institution

Samples

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Human Th0 cell clone, 0 min, biological rep4
peripheral blood
none
0 min
Human Tr1 cell clone, 0 min, biological rep2
peripheral blood
none
0 min
Human Th0 cell clone,16h, biological rep1
peripheral blood
stimulated with immobilized anti-cd3 and soluble anti-cd28 mabs
16h
Human Th0 cell clone, 6h, biological rep5
peripheral blood
stimulated with immobilized anti-cd3 and soluble anti-cd28 mabs
6h
Human Tr1 cell clone, 0 min, biological rep3
peripheral blood
none
0 min
Human Th0 cell clone, 0 min, biological rep11
peripheral blood
none
0 min
Human Tr1 cell clone, 0 min, biological rep5
peripheral blood
none
0 min
Human Tr1 cell clone, 0 min, biological rep4
peripheral blood
none
0 min
Human Th0 cell clone, 0 min, biological rep7
peripheral blood
none
0 min
Human Th0 cell clone, 6h,biological rep4
peripheral blood
stimulated with immobilized anti-cd3 and soluble anti-cd28 mabs
6h
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