"Total RNA was extracted from cells using a combination of the trizol protocol and the RNAeasy mini kit (Qiagen, #74106). Poly A RNA was extracted using the Poly (A) Purist Mag kit (Ambion # AM 1992). ABI whole transcriptome library preparation kit (#4425680) was used to generate the RNA-Seq libraries."

AB SOLiD System 3.0 sequencing; RNA-Seq of human ESC cell line in monolayer culture: Sample Day0hESC_Monolayer

Homo sapiens

Use traditional whole transcriptome profiling, and single cell whole transcriptome profiling to understand human pre-implantation development, undifferentiated human embryonic stem cells and differentiated human embryonic stem cells.

Homo sapiens Transcriptome or Gene expression

Submitter Supplied Information

Samples