Illumina HiSeq 1000 paired end sequencing; GSM2327091: E16.5_ProC_01; Mus musculus; RNA-Seq

E16.5_ProC_01

Single-cell RNA-seq analysis of pre- and postnatal mouse endolymphatic sac demonstrates two types of differentiated cells distinguished by their mRNA expression signatures. Overall design: mRNA-seq profiles from 213 single cells from embryonic day 12.5, 16.5, postnatal day 5 and 30 mouse endolymphatic sac were analyzed

Molecular architecture underlying fluid absorption by the developing inner ear

Submitted by Gene Expression Omnibus on 25-SEP-2017

Following dissociation of tissue into a single-cell suspension, target cells were enriched by purification as described, cells were lysed, total RNA reverse-transcribed with oligo-dT primers, and cDNA amplified using SMARTer ultra-low input cDNA synthesis (Clontech) on the C1 microfluidics platform (Fluidigm). cDNA libraries were prepared for sequencing using Nextera XT tagmentation and 96-barcode indexing (Illumina). Barcoded samples were pooled, cleaned with Agencourt AMPure XP magnetic bead isolation (Beckman Coulter).

Molecular architecture underlying fluid absorption by the developing inner ear

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