mRNA sequencing to assess RfxCasR and matching shRNA specificity

SRP127547

Homo sapiens

21 Downloadable Samples

NextSeq 500

Submitter Supplied Information

Description

Matching sets of RfxCasR and shRNAs targeting ANXA4 and B4GALNT1 plus non-targeting (NT) controls were profiled by mRNA sequencing to compare non-specific transcriptome perturbations for both shRNA and RfxCasR technologies. Overall design: Three biological replicates for 3 shRNAs and 2 RfxCasR guide RNAs plus 2 RfxCasR arrays expresssed in HEK 293FT cells

PubMed ID

29551272

Publication Title

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.

Total Samples

Submitter’s Institution

No associated institution

Authors

Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD

Source Repository

Sequence Read Archive (SRA)

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