Potential role of gas6 in zebrafish hindbrain development

Build and Download Custom Datasets

refine.bio helps you build ready-to-use datasets with normalized transcriptome data from all of the world’s genetic databases.

SRP141198

Danio rerio

6 Downloadable Samples

Illumina HiSeq 2000

Submitter Supplied Information

Description

identification of differentially expressed genes in gas6 homozygous mutant hindbrain when compared to wildtype hindbrain in zebrafish Overall design: Total RNA was extracted from dissected hindbrain of gas6 homzygous mutants and wildtype embryos at 48hpf using the RNeasy Mini Kit (Qiagen). Three libraries from wildtype embryos and three libraries from gas6 mutants were then generated from 3mg RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina). All libraries were analyzed for quality on a bioanalyzer prior to sequencing (Agilent 2100 BioAnalyzer).

PubMed ID

29945667

Publication Title

Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish.

Total Samples

Submitter’s Institution

No associated institution

Authors

Ghosh P, Maurer JM, Sagerström CG

Source Repository

Sequence Read Archive (SRA)

No rows found