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Homo sapiens
6 Downloadable Samples
Illumina HiSeq 2500
Description
RNA-seq analysis of 6 WHO grade-II tumors (n=4 with the rs55705857 genotype A/G and n=2 with the genotype A/A) that were IDH1-R132H mutant, 1p/19q co-deleted and ATRX-wild-type.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Race
View Samples- Homo sapiens
- 12 Downloadable Samples
Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
ELABELA (ELA) is a peptide hormone required for heart development that signals via the Apelin Receptor (APLNR, APJ). ELA is also abundantly secreted by human embryonic stem cells (hESCs), which do not express APLNR. Here we show that ELA signals in a paracrine fashion in hESCs to maintain self-renewal. ELA inhibition by CRISPR/Cas9-mediated deletion, shRNA or neutralizing antibodies causes reduced hESC growth, cell death and loss of pluripotency. Global phosphoproteomic and transcriptomic analyses of ELA-pulsed hESCs show that it activates PI3K/AKT/mTORC1 signaling required for cell survival. ELA promotes hESC cell cycle progression and protein translation, and blocks stress-induced apoptosis. INSULIN and ELA have partially overlapping functions in hESC medium, but only ELA can potentiate the TGF pathway to prime hESCs towards the endoderm lineage. We propose that ELA, acting through an alternate cell-surface receptor, is an endogenous secreted growth factor in human embryos and hESCs that promotes growth and pluripotency.
Publication Title
ELABELA Is an Endogenous Growth Factor that Sustains hESC Self-Renewal via the PI3K/AKT Pathway.
Sample Metadata Fields
Specimen part, Treatment
View Samples- Homo sapiens
- 12 Downloadable Samples
- Illumina HumanHT-12 V4.0 expression beadchip
Description
This study identifies miR-198 as a potential inhibitor of keratinocyte migration in skin
Publication Title
'See-saw' expression of microRNA-198 and FSTL1 from a single transcript in wound healing.
Sample Metadata Fields
Specimen part, Time
View Samples- Homo sapiens
- 54 Downloadable Samples
Description
AML patient samples and a few normal blood sample were assayed for H3K27ac ChIP-seq and RNA-seq. We discovered subtypes of AML based on these enhancer landscapes.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Caenorhabditis elegans
- 26 Downloadable Samples
- Illumina HiSeq 2500
Description
LITE-Seq 3'' end capture and sequencing of the C. elegans germline
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Arabidopsis thaliana
- 16 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
This microarray experiment serves to identify the genes in the Arabidopsis genome that are regulated by carbon and light signaling interactions in 7 day dark grown seedlings. The expression profile of wild-type will be compared to the cli186 mutant, a mutant defective in carbon and light signaling. Plants of both the wild-type and cli186 genotypes are treated with the following light (L) and carbon (C) treatments: -C-L, +C-L, +C+L, -C+L. Comparison of the expression profiles under all treatments will help to identify genes that are misregulated in carbon and/or light treatments in the cli186 mutant.
Publication Title
An integrated genetic, genomic and systems approach defines gene networks regulated by the interaction of light and carbon signaling pathways in Arabidopsis.
Sample Metadata Fields
Age
View Samples- Danio rerio
- 12 Downloadable Samples
- IlluminaHiSeq2500
Description
Heart tissue was enriched from 48hpf zebrafish larvae from different experimental conditions. Approximately 200 hearts were collected for each sample. The goals of the study were to profile transcriptional outputs in the cardiac tissue which affects heart development between two different experimental conditions.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples
SRP105243- Danio rerio
- 12 Downloadable Samples
- IlluminaHiSeq2000
Description
The goal of the experiment was to simulate the effect of size-selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 10 Downloadable Samples
- Illumina HiSeq 2500
Description
Study Goals: To understand the impact of macrophage retention of exosomes on macrophage gene expression and function. Additionally, we also wanted to understand the effect of TLR3 stimulation of HEY cells on exosome mediated macrophage functionsRelevance: Exosomes have a multitude of effects in immunomodulation and cancer cells secrete exosomes that alter the fate of cancer progression. In this study we seek to: 1) understand the effect of TLR stimulation on local cells producing exosomes2) understand the impact of cancer secreted exosomes on cancer progression.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line, Treatment
View Samples- Mus musculus
- 10 Downloadable Samples
- Illumina HiSeq 2500
Description
Study goals:To understand the impact of sustained lymph node retention of exosomes on whole lymph node gene expression and function.Relevance: Exosomes have a multitude of effects in immunomodulation and cancer cells secrete exosomes that alter the fate of cancer progression. In this study we seek to: 1) understand the effect of TLR stimulation on local cells producing exosomes 2) understand the impact of cancer secreted exosomes on cancer progression.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line, Treatment
View Samples