Gene expression profiles of bitter TRCs and profiles of sweet and umami TRCs.
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Sex, Age, Specimen part, Cell line
View SamplesNKTp, NKT1, NKT2, NKT17 were flow sorted from 7 wk old female live BALB/c TBET (gfp) KN2 reporter mice and RNA sequenced
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Sex, Specimen part
View SamplesCompared differentially express genes by sex in mouse for the following tissues: hypothalamus, liver, kidney, ovaries and testis (3 biological x 2 technical replicates for each tissues/sex). We used Affymetrix MOE430A Genechip arrays.
Major molecular differences between mammalian sexes are involved in drug metabolism and renal function.
Sex, Specimen part
View SamplesmRNA sequencing of PyMT primary tumor cells with and without E-cadherin were isolated using FACS, based on expression of tumor cell-specific YFP expression, and sorted into an E-cadherin-positive and E-cadherin-negative population based on the expression of endogenous E-cadherin-mCFP.
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View SamplesIn this study, we aimed to identify potential lncRNA deregulations associated with breast cancer malignancy instigated by MSCs-MCF7 co-culture. We profiled expression changes of lncRNAs in MCF-7 cells during EMT induced by coculture with hAD-MSCs, and found an intergenic lncRNA with proviouly unknown function (KB-1732A1.1, we termed it LincK), which was significantly elevated. Depletion of LincK decreased the growth, migration, invasion, and EMT in breast cancer cells, while overexpression of LincK exerted the opposite effects. Moreover, knockdown of LincK repressed tumorigenesis, and ectopic expression of LincK promoted tumor growth in MCF-7 xenograft model. LincKs can act as a sponge of mirR-200b, which inhibits its function in proliferation and metastasis. Thus we reported, for the rst time, the role of LincK in control of EMT and proliferation in breast cancer.
LincK contributes to breast tumorigenesis by promoting proliferation and epithelial-to-mesenchymal transition.
Cell line
View SamplesHeLa cell culture RNASeq data was obtained to demonstrate the effectiveness of the Cas9 based DASH technique for depletion of unwanted abundant sequences.
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View SamplesInvestigation of differentially expressed genes in circZbtb20 or Nr4a1 deficient ILC3s
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View SamplesInvestigation of differentially expressed genes in Ccp6-depleted D3 ESCs
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View SamplesInvestigation of differentially expressed genes in Ccp2-deficient CHILPs
IL-7Rα glutamylation and activation of transcription factor Sall3 promote group 3 ILC development.
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View SamplesInvestigation of differentially expressed genes in circKcnt2-deficient ILC3s
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View Samples